Description:

Library Preparation for Illumina

It’s complex, but we have it covered.

KAPA DNA Library Preparation Kits for Illumina^® sequencing platforms contain evolved and optimally formulated enzymes that enable the highest overall coverage from the least amount of total sequencing.

Kits are optimized to achieve significantly higher library yields and contain KAPA HiFi for high-fidelity, high-efficiency and low-bias library amplification. This ensures the highest library and sequence data quality, particularly from low-input and difficult samples such as FFPE and ChIP DNA.

NEW! KAPA Dual-Indexed Adapter Kits are now available. For more information on KAPA Adapter Kits, scroll down to the Ordering section, or download the KAPA Adapter and Bead Calculator.

Download our KAPA  Adapter and Bead Calculator

For Research Use Only. Not for use in diagnostic procedures.

Product Highlights

Achieve greater molecular complexity

• Higher yields of adapter-ligated library translates to higher molecular complexity
• Fewer cycles of amplification are needed, which results in lower PCR duplication rates
• PCR-free workflows possible from inputs ≥100 ng*

Reduce amplification bias and improve coverage

• KAPA HiFi reduces PCR bias*
• Improves coverage uniformity of GC- and AT-rich regions, promoters, low complexity and other challenging regions*

Create high-quality libraries from challenging samples

• High-quality whole exome sequencing from libraries prepared using as little as 10 ng human genomic DNA*
• High success rates with 250 ng FFPE or less*
• Routine library construction from ≥100 pg ChIP DNA*

Automate with pre-validated scripts

• PerkinElmer Sciclone^® NGS and NGSx
• Beckman Coulter^® Biomek™ FXp and FX dual-pod
• Agilent^® Technologies NGS Workstation Option B
• Eppendorf^® epMotion™ 5075 TMX

_{*Data on file.}

Applications:

• Whole Genome Sequencing
• ChIP Sequencing
• Whole Exome Sequencing
• Amplicon Sequencing
• Targeted Sequencing (Capture)
• Methyl Sequencing

Kit Specifications and Contents / Storage:

Kits can be stored for up to 18 months at -20˚C.

Kits include reagents for end repair, A-tailing, ligation and library amplification. PCR-free versions (without library amplification reagent) are also available. \"With-bead” kits include PEG/NaCl solution for SPRI bead cleanups.All 96-library kits are automation friendly.

Components

Specifications